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Analysis of institutional authors

Chou, Yu FuAuthorMarti, Juan Manuel NunezAuthorSauro, SalvatoreCorresponding Author

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November 26, 2024
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Biocompatibility and osteogenic assessment of experimental fluoride-doped calcium-phosphate cements on human dental pulp stem cells

Publicated to: Dental Materials. 40 (11): 2043-2050 - 2024-11-13 40(11), DOI: 10.1016/j.dental.2024.09.019

Authors:

Del Giudice, C; Spagnuolo, G; Menale, C; Chou, YF; Martí, JMN; Rengo, C; Rengo, S; Sauro, S
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Affiliations

Cardenal Herrera CEU Univ, CEU Univ, Fac Hlth Sci, Dept Dent,Dent Biomat & Minimally Invas Dent, Valencia, Spain - Author
Univ Naples Federico II, Dept Clin Med & Surg, I-80131 Naples, Italy - Author
Univ Naples Federico II, Dept Neurosci Reprod & Odontostomatol Sci, I-80131 Naples, Italy - Author
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Abstract

Objectives: This study investigated the impact of some specific experimental calcium phosphate cements doped with different fluoride salts (FDCPCs) concentrations on the basal functions of human Dental Pulp Stem Cells (hDPSCs). Furthermore, this study also examined the migration, as well as the mineralisation through osteogenic differentiation. Methods: Experimental FDCPCs were formulated using different concentrations of calcium/sodium fluoride salts [(5 wt%: VS5F), (10 wt%: VS10F), (20 wt%: VS20F)]. A fluoride-free calcium phosphate (VS0F) was used as a control. The hDPSCs were assessed to evaluate their self-renewal and migration activity in the presence of eluates of the different FDCPCs. A viability assay in osteogenic conditions was carried out, along with the differentiation potential through Alkaline Phosphatase Activity (ALP), and Alizarin Red Staining (ARS). Moreover, the gene expression of specific markers (RUNX2, ALP, COL1 alpha 1, OCN, OPN, DSPP, MEPE, and DMP-1) was also evaluated. Results: All the tested FDCPD had no influence on cell migrations, but they caused a decrease in cell viability in osteogenic conditions when not diluted. Conversely, the eluants of VS20F showed a positive effect on stem cell differentiation. This result was corroborated through ALP activity, ARS assay. Moreover, upregulation of specific gene markers such as RUNX2, DMP-1, and DSPP was observed in hDPSCs, especially when treated with VS20F. Significance: The experimental FDCPC tested in this study exhibits a dose-dependent capacity to promote mineralisation in osteogenic environment. The FDCPC-VS20F seems to be the most promising experimental material suitable for developing of pulp-capping materials with osteogenic and bioactive properties.
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Keywords

Alkaline phosphataseBioactive glassesBiocompatibilityBiocompatible materialsBiodentineCalcium phosphateCalcium phosphatesCapping materialsCell differentiationCell movementCell survivalCells, culturedCytotoxicityDental cementsDental pulpDifferentiationFluoridesHuman dental pulp stem cellsHumansIn-vitro evaluationIon-releaseMaterials testingOsteogenesisOsteogenicProliferationReleasRemineralisation markerRemineralisation markersRemineralizationStem cellsStrategies

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Dental Materials due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position 6/162, thus managing to position itself as a Q1 (Primer Cuartil), in the category Dentistry, Oral Surgery & Medicine. Notably, the journal is positioned above the 90th percentile.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-12-28:

  • WoS: 7
  • Scopus: 9
  • Europe PMC: 1
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-12-28:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 17 (PlumX).
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Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Italy.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: Last Author (Sauro, Salvatore).

the author responsible for correspondence tasks has been Sauro, Salvatore.

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Awards linked to the item

All authors gave their final approval and agreed to be accountable for all aspects of the work. There is a patent on the calcium phosphates tailored with different concentrations of fluoride salts used in this study (ES2716942-Composicion y procedimiento para la obtencion y aplicacion de un compuesto bioactivo que contiene fluoruro y el pro-ducto obtenido) . The authors have no financial affiliation or involve-ment with any commercial organisation with a direct financial interest in the materials discussed in this manuscript. This study was supported by the grant PID2020-120346GB-I00 funded by AEI/10.13039/501100011033 "Ministerio de Ciencia, Innovacion y Universidades."
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