Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

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Work in the laboratory of Dieter Adam is supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)-Projektnummer 125440785 - SFB 877, Project B2.

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October 28, 2019

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Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Publicated to:European Journal Of Immunology. 49 (10): 1457-1973 - 2019-10-01 49(10), DOI: 10.1002/eji.201970107

Authors: Cossarizza, A; Chang, HD; Radbruch, A; Acs, A; Adam, D; Adam-Klages, S; Agace, WW; Aghaeepour, N; Akdis, M; Allez, M; Almeida, LN; Alvisi, G; Anderson, G; Andrä, I; Annunziato, F; Anselmo, A; Bacher, P; Baldari, CT; Bari, S; Barnaba, V; Barros-Martins, J; Battistini, L; Bauer, W; Baumgart, S; Baumgarth, N; Baumjohann, D; Baying, B; Bebawy, M; Becher, B; Beisker, W; Benes, V; Beyaert, R; Blanco, A; Boardman, DA; Bogdan, C; Borger, JG; Borsellino, G; Boulais, PE; Bradford, JA; Brenner, D; Brinkman, RR; Brooks, AES; Busch, DH; Büscher, M; Bushnell, TP; Calzetti, F; Cameron, G; Cammarata, I; Cao, X; Cardell, SL; Casola, S; Cassatella, MA; Cavani, A; Celada, A; Chatenoud, L; Chattopadhyay, PK; Chow, S; Christakou, E; Cicin-Sain, L; Clerici, M; Colombo, FS; Cook, L; Cooke, A; Cooper, AM; Corbett, AJ; Cosma, A; Cosmi, L; Coulie, PG; Cumano, A; Cvetkovic, L; Dang, VD; Dang-Heine, C; Davey, MS; Davies, D; De Biasi, S; Del Zotto, G; Dela Cruz, GV; Delacher, M; Della Bella, S; Dellabona, P; Deniz, G; Dessing, M; Di Santo, JP; Diefenbach, A; Dieli, F; Dolf, A; Dörner, T; Dress, RJ; Dudziak, D; Dustin, M; Dutertre, CA; Ebner, F; Eckle, SBG; Edinger, M; Eede, P; Ehrhardt, GRA; Eich, M; Engel, P; Engelhardt, B; Erdei, A; Esser, C; Everts, B; Evrard, M; Falk, CS; Fehniger, TA; Felipo-Benavent, M; Ferry, H; Feuerer, M; Filby, A; Filkor, K; Fillatreau, S; Follo, M; Förster, I; Foster, J; Foulds, GA; Frehse, B; Frenette, PS; Frischbutter, S; Fritzsche, W; Galbraith, DW; Gangaev, A; Garbi, N; Gaudilliere, B; Gazzinelli, RT; Geginat, J; Gerner, W; Gherardin, NA; Ghoreschi, K; Gibellini, L; Ginhoux, F; Goda, K; Godfrey, DI; Goettlinger, C; González-Navajas, JM; Goodyear, CS; Gori, A; Grogan, JL; Grummitt, D; Grützkau, A; Haftmann, C; Hahn, J; Hammad, H; Hämmerling, G; Hansmann, L; Hansson, G; Harpur, CM; Hartmann, S; Hauser, A; Hauser, AE; Haviland, DL; Hedley, D; Hernández, DC; Herrera, G; Herrmann, M; Hess, C; Höfer, T; Hoffmann, P; Hogquist, K; Holland, T; Höllt, T; Holmdahl, R; Hombrink, P; Houston, JP; Hoyer, BF; Huang, B; Huang, FP; Huber, JE; Huehn, J; Hundemer, M; Hunter, CA; Hwang, WYK; Iannone, A; Ingelfinger, F; Ivison, SM; Jäck, HM; Jani, PK; Jávega, B; Jonjic, S; Kaiser, T; Kalina, T; Kamradt, T; Kaufmann, SHE; Keller, B; Ketelaars, SLC; Khalilnezhad, A; Khan, S; Kisielow, J; Klenerman, P; Knopf, J; Koay, HF; Kobow, K; Kolls, JK; Kong, WT; Kopf, M; Korn, T; Kriegsmann, K; Kristyanto, H; Kroneis, T; Krueger, A; Kühne, J; Kukat, C; Kunkel, D; Kunze-Schumacher, H; Kurosaki, T; Kurts, C; Kvistborg, P; Kwok, I; Landry, J; Lantz, O; Lanuti, P; LaRosa, F; Lehuen, A; LeibundGut-Landmann, S; Leipold, MD; Leung, LYT; Levings, MK; Lino, AC; Liotta, F; Litwin, V; Liu, Y; Ljunggren, HG; Lohoff, M; Lombardi, G; Lopez, L; López-Botet, M; Lovett-Racke, AE; Lubberts, E; Luche, H; Ludewig, B; Lugli, E; Lunemann, S; Maecker, HT; Maggi, L; Maguire, O; Mair, F; Mair, KH; Mantovani, A; Manz, RA; Marshall, AJ; Martínez-Romero, A; Martrus, G; Marventano, I; Maslinski, W; Matarese, G; Mattioli, AV; Maueröder, C; Mazzoni, A; McCluskey, J; McGrath, M; McGuire, HM; McInnes, IB; Mei, HE; Melchers, F; Melzer, S; Mielenz, D; Miller, SD; Mills, KHG; Minderman, H; Mjösberg, J; Moore, J; Moran, B; Moretta, L; Mosmann, TR; Müller, S; Multhoff, G; Muñoz, LE; Münz, C; Nakayama, T; Nasi, M; Neumann, K; Ng, LG; Niedobitek, A; Nourshargh, S; Núñez, G; O'Connor, JE; Ochel, A; Oja, A; Ordonez, D; Orfao, A; Orlowski-Oliver, E; Ouyang, W; Oxenius, A; Palankar, R; Panse, I; Pattanapanyasat, K; Paulsen, M; Pavlinic, D; Penter, L; Peterson, P; Peth, C; Petriz, J; Piancone, F; Pickl, WF; Piconese, S; Pinti, M; Pockley, AG; Podolska, MJ; Poon, Z; Pracht, K; Prinz, I; Pucillo, CEM; Quataert, SA; Quatrini, L; Quinn, KM; Radbruch, H; Radstake, TRDJ; Rahmig, S; Rahn, HP; Rajwa, B; Ravichandran, G; Raz, Y; Rebhahn, JA; Recktenwald, D; Reimer, D; Reis e Sousa, C; Remmerswaal, EBM; Richter, L; Rico, LG; Riddell, A; Rieger, AM; Robinson, JP; Romagnani, C; Rubartelli, A; Ruland, J; Saalmüller, A; Saeys, Y; Saito, T; Sakaguchi, S; Sala-de-Oyanguren, F; Samstag, Y; Sanderson, S; Sandrock, I; Santoni, A; Sanz, RB; Saresella, M; Sautes-Fridman, C; Sawitzki, B; Schadt, L; Scheffold, A; Scherer, HU; Schiemann, M; Schildberg, FA; Schimisky, E; Schlitzer, A; Schlosser, J; Schmid, S; Schmitt, S; Schober, K; Schraivogel, D; Schuh, W; Schüler, T; Schulte, R; Schulz, AR; Schulz, SR; Scottá, C; Scott-Algara, D; Sester, DP; Shankey, TV; Silva-Santos, B; Simon, AK; Sitnik, KM; Sozzani, S; Speiser, DE; Spidlen, J; Stahlberg, A; Stall, AM; Stanley, N; Stark, R; Stehle, C; Steinmetz, T; Stockinger, H; Takahama, Y; Takeda, K; Tan, L; Tárnok, A; Tiegs, G; Toldi, G; Tornack, J; Traggiai, E; Trebak, M; Tree, TIM; Trotter, J; Trowsdale, J; Tsoumakidou, M; Ulrich, H; Urbanczyk, S; van de Veen, W; van den Broek, M; van der Pol, E; Van Gassen, S; Van Isterdael, G; van Lier, RAW; Veldhoen, M; Vento-Asturias, S; Vieira, P; Voehringer, D; Volk, HD; von Borstel, A; von Volkmann, K; Waisman, A; Walker, RV; Wallace, PK; Wang, SA; Wang, XM; Ward, MD; Ward-Hartstonge, KA; Warnatz, K; Warnes, G; Warth, S; Waskow, C; Watson, JV; Watzl, C; Wegener, L; Weisenburger, T; Wiedemann, A; Wienands, J; Wilharm, A; Wilkinson, RJ; Willimsky, G; Wing, JB; Winkelmann, R; Winkler, TH; Wirz, OF; Wong, A; Wurst, P; Yang, JHM; Yang, JH; Yazdanbakhsh, M; Yu, LP; Yue, A; Zhang, HL; Zhao, Y; Ziegler, SM; Zielinski, C; Zimmermann, J; Zychlinsky, A

Affiliations

Aix Marseille Univ, Ctr Immunophenom CIPHE PHENOMIN, UMS3367, Inserm,US012,CNRS,UMS3367, Marseille, France - Author

Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10467 USA - Author

Albert Einstein Coll Med, Dept Med, Bronx, NY 10467 USA - Author

Alicante Inst Hlth & Biomed Res ISABIAL, Alicante, Spain - Author

Amgen Inc, Inflammat & Oncol, Res, San Francisco, CA USA - Author

and Pediatrics, Stanford University, Stanford, CA, USA. - Author

APE Appl Phys & Elect, Berlin, Germany - Author

AsedaSciences, W Lafayette, IN USA - Author

ASTAR, Singapore Immunol Network SIgN, Biopolis, Singapore, Singapore - Author

Australian Research Council Centre of Excellence in Advanced Molecular Imaging, Monash University, Clayton, Victoria, Australia. - Author

Babraham Inst, Flow Cytometry Facil, Cambridge, England - Author

BC Children's Hospital Research Institute, Vancouver, Canada. - Author

BC Childrens Hosp Res Inst, Vancouver, BC, Canada - Author

BD Life Sci, Ashland, OR USA - Author

BD Life Sci, San Diego, CA USA - Author

Beckman Coulter Inc, Miami, FL USA - Author

Berlin Inst Hlth BIH, Berlin, Germany - Author

Berlin Inst Hlth, Berlin, Germany - Author

Berlin Inst Hlth, Core Unit ImmunoCheck, Berlin, Germany - Author

Berlin Inst Hlth, Dept Dermatol Venereol & Allergol, Berlin, Germany - Author

Berlin Inst Hlth, Dept Neuropathol, Berlin, Germany - Author

Berlin Inst Hlth, Dept Rheumatol & Clin Immunol, Berlin, Germany - Author

Berlin Inst Hlth, Inst Med Immunol, Berlin, Germany - Author

Berlin Institute of Health (BIH), Berlin, Germany. - Author

BioGenes GmbH, Berlin, Germany - Author

Biophysics, R&D Engineering, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany. - Author

BSRC Alexander Fleming, Inst Bioinnovat, Vari, Greece - Author

Burnet Inst, AMREP Flow Cytometry Core Facil, Melbourne, Vic, Australia - Author

Cancer & Stem Cell Biology, Duke-NUS Medical School, Singapore. - Author

Catholic Univ Louvain, De Duve Inst, Brussels, Belgium - Author

Center for Comparative Medicine & Dept. Pathology, Microbiology & Immunology, University of California, Davis, CA, USA. - Author

Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Rome, Italy. - Author

Chair for Immunology, University Regensburg, Germany. - Author

Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Neuropathology, Germany. - Author

Charité - Universitätsmedizin Berlin, Laboratory of Innate Immunity, Department of Microbiology, Infectious Diseases and Immunology, Berlin, Germany. - Author

Charite Univ Med Berlin, BCRT Flow Cytometry Lab, Berlin Brandenburg Ctr Regenerat Therapies, Berlin, Germany - Author

Charite Univ Med Berlin, BCRT, Berlin, Germany - Author

Charite Univ Med Berlin, Berlin, Germany - Author

Charite Univ Med Berlin, Clin Res Unit, BIH, Berlin, Germany - Author

Charite Univ Med Berlin, Dept Dermatol Venereol & Allergol, Berlin, Germany - Author

Charite Univ Med Berlin, Dept Hematol Oncol & Tumor Immunol, Campus Virchow Klinikum, Berlin, Germany - Author

Charite Univ Med Berlin, Dept Med Rheumatol & Clin Immunol, Berlin, Germany - Author

Charite Univ Med Berlin, Dept Microbiol Infect Dis & Immunol, Lab Innate Immun, Berlin, Germany - Author

Charite Univ Med Berlin, Flow & Mass Cytometry Core Facil, Berlin, Germany - Author

Charite Univ Med Berlin, Med Dept 1, Div Gastroenterol Infectiol & Rheumatol, Berlin, Germany - Author

Charles Univ Prague, Fac Med 2, Dept Paediat Haematol & Oncol, Prague, Czech Republic - Author

Chiba Univ, Dept Immunol, Grad Sch Med, Chiba, Chiba, Japan - Author

Chinese Acad Med Sci & Peking Union Med Coll, Dept Immunol, Beijing, Peoples R China - Author

Chinese Acad Med Sci & Peking Union Med Coll, Natl Key Lab Med Mol Biol, Inst Basic Med Sci, Beijing, Peoples R China - Author

Chinese Acad Med Sci & Peking Union Med Coll, State Key Lab Expt Hematol, Inst Hematol, Tianjin, Peoples R China - Author

Christian Albrechts Univ Kiel, Inst Immunol, Kiel, Germany - Author

Christian Albrechts Univ Kiel, Inst Klin Mol Biol, Kiel, Germany - Author

Christine Kuhne Ctr Allergy Res & Educ CK CARE, Davos, Switzerland - Author

Chromocyte Ltd, Elect Works, Sheffield, S Yorkshire, England - Author

Clinical Research Unit, Berlin Institute of Health (BIH), Charite Universitätsmedizin Berlin, Berlin, Germany. - Author

CNR, IEOS, Naples, Italy - Author

Comprehens Canc Ctr Zurich, Zurich, Switzerland - Author

Comprehensive Cancer Center Zurich, Switzerland. - Author

Ctr Rech Cordeliers, Equipe Canc & Immunite Antitumorale, Paris, France - Author

de Duve Institute, Université catholique de Louvain, Brussels, Belgium. - Author

Department of Biology, Nikolaus-Fiebiger-Center for Molecular Medicine, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany. - Author

Department of Biomedical Molecular Biology, Center for Inflammation Research, Ghent University - VIB, Ghent, Belgium. - Author

Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY, USA. - Author

Department of Dermatology, Laboratory of Dendritic Cell Biology, Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), University Hospital Erlangen, Erlangen, Germany. - Author

Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy. - Author

Department of Immunobiology, School of Immunology and Microbial Sciences, King's College London, UK. - Author

Department of Immunology and Pathology, Monash University, Melbourne, Victoria, Australia. - Author

Department of Immunology, University L. Eotvos, Budapest, Hungary. - Author

Department of Immunology, University of Toronto, Toronto, ON, Canada. - Author

Department of Internal Medicine III, University Hospital Regensburg, Germany. - Author

Department of Life Sciences, University of Siena, Siena, Italy. - Author

Department of Medical and Surgical Sciences for Children and Adults, Univ. of Modena and Reggio Emilia School of Medicine, Modena, Italy. - Author

Department of Medical Biotechnologies and Translational Medicine, University of Milan, Milan, Italy. - Author

Department of Medical Genetics, University of British Columbia, Vancouver, BC, Canada. - Author

Department of Medicine, The University of British Columbia, Vancouver, Canada. - Author

Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Parkville, Victoria, Australia. - Author

Department of Microbiology and Immunology, University of Gothenburg, Gothenburg, Sweden. - Author

Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands. - Author

Department of Pathology, University of Cambridge, Cambridge, UK. - Author

Department of Pediatrics and Shared Resource Laboratories, University of Rochester Medical Center, Rochester, NY, USA. - Author

Department of Physiopathology and Transplants, University of Milan, Milan, Italy. - Author

Department of Respiratory Sciences, University of Leicester, Leicester, UK. - Author

Department of Surgery, Medicine, Dentistry and Morphological Sciences, Univ. of Modena and Reggio Emilia, Modena, Italy. - Author

Department of Surgery, The University of British Columbia, Vancouver, Canada. - Author

Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, Braunschweig, Germany. - Author

Departments of Anesthesiology, Pain and Perioperative Medicine, Biomedical Data Sciences - Author

Dept. Medicine/Rheumatology and Clinical Immunology, Charité Universitätsmedizin Berlin, Germany. - Author

Desatoya LLC, Reno, NV USA - Author

Deutsch Rheuma Forschungszentrum DRFZ, Berlin, Germany - Author

Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany. - Author

Dipartimento di Medicina Interna e Specialita Mediche, Sapienza Universita di Roma, Rome, Italy. - Author

Discipline of Pharmacy, Graduate School of Health, The University of Technology Sydney, Sydney, NSW, Australia. - Author

Division of Immunology, Allergy and Infectious Diseases, Department of Dermatology, Medical University of Vienna, Vienna, Austria. - Author

Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Milan, Italy. - Author

Division of Medical Sciences, National Cancer Centre Singapore, Singapore. - Author

Division of Molecular Immunology, Nikolaus-Fiebiger-Center, Dept. of Internal Medicine III, University of Erlangen-Nuremberg, Erlangen, Germany. - Author

Division of Oncology, Washington University School of Medicine, St. Louis, MO, USA. - Author

Divsion of Medical Oncology and Hematology, Princess Margaret Hospital, Toronto, Ontario, Canada. - Author

Duke NUS Med Sch, Program Emerging Infect Dis, Singapore, Singapore - Author

EMBL, Flow Cytometry Core Facil, Heidelberg, Germany - Author

EMBL, Genom Core Facil, Heidelberg, Germany - Author

EMBL, Genome Biol Unit, Heidelberg, Germany - Author

Experimental Medicine Division, Nuffield Department of Medicine, University of Oxford, Oxford, UK. - Author

Fac Med & Hlth Sci, Dept Internal Med & Pediat, Zwijnaarde, Belgium - Author

Fdn Cenci Bolognetti, Ist Pasteur, Rome, Italy - Author

Fdn Ist Nazl Genet Mol Ronmeo & Enric Invernizzi, INGM, Milan, Italy - Author

Fdn Santa Lucia IRCCS, Flow Cytometry Unit, Rome, Italy - Author

Fdn Santa Lucia IRCCS, Neuroimmunol Unit, Rome, Italy - Author

FIRC Inst Mol Oncol FOM, Milan, Italy - Author

Flow Cytometry Core Facility, Institute of Experimental Immunology, University of Bonn, Bonn, Germany. - Author

Flow Cytometry Core Technologies, UCD Conway Institute, University College Dublin, Dublin, Ireland. - Author

Flow Cytometry Core, Humanitas Clinical and Research Center, Milan, Italy. - Author

Flow Cytometry Laboratory, Institute of Molecular Toxicology and Pharmacology, Helmholtz Zentrum München, German Research Center for Environmental Health, München, Germany. - Author

Flow Cytometry Scientific Technology Platform, The Francis Crick Institute, London, UK. - Author

Focus Group ''Clinical Cell Processing and Purification", Institute for Advanced Study, Technische Universität München, Munich, Germany. - Author

Francis Crick Inst, Flow Cytometry Sci Technol Platform, London, England - Author

Francis Crick Inst, Immunobiol Lab, London, England - Author

Fraunhofer Inst Cell Therapy & Immunol IZI, Dept Therapy Validat, Leipzig, Germany - Author

Fred Hutchinson Canc Res Ctr, Vaccine & Infect Dis Div, 1124 Columbia St, Seattle, WA 98104 USA - Author

Free Univ Berlin, Berlin, Germany - Author

Free Univ Berlin, Inst Immunol, Ctr Infect Med, Dept Vet Med, Berlin, Germany - Author

Friedrich Alexander Univ Erlangen Nuremberg, Dept Biol, Nikolaus Fiebiger Ctr Mol Med, Erlangen, Germany - Author

Friedrich Alexander Univ Erlangen Nurnberg FAU, Dept Infect Biol, Univ Hosp Erlangen, Erlangen, Germany - Author

Friedrich Alexander Univ Erlangen Nurnberg FAU, Dept Med Rheumatol & Immunol 3, Univ Klinikum Erlangen, Erlangen, Germany - Author

Friedrich Alexander Univ FAU Erlangen Nurnberg, Erlangen, Germany - Author

Friedrich Schiller Univ Jena, Fac Biol Sci, Jena, Germany - Author

Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Medical Immunology Campus Erlangen, Erlangen, Germany. - Author

Fritz Lipmann Inst FLI, Regenerat Hematopoiesis, Leibniz Inst Aging, Jena, Germany - Author

Fundacao Oswaldo Cruz Minas, Lab Immunopatol, Belo Horizonte, MG, Brazil - Author

Genentech Inc, Canc Immunol Res, San Francisco, CA USA - Author

Genomics Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany. - Author

German Canc Consortium DKTK, Partner Site Berlin, Berlin, Germany - Author

German Canc Res Ctr, Div Theoret Syst Biol, Heidelberg, Germany - Author

German Canc Res Ctr, Flow Cytometry Core Facil, Heidelberg, Germany - Author

German Canc Res Ctr, Heidelberg, Germany - Author

German Center for Infection Research (DZIF), Munich, Germany. - Author

German Ctr Infect Res DZIF, Munich, Germany - Author

German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Inst Mol Toxicol & Pharmacol, Flow Cytometry Lab, Munich, Germany - Author

Ghent Univ VIB, Dept Biomed Mol Biol, Ctr Inflammat Res, Ghent, Belgium - Author

Goethe Univ Frankfurt, Inst Mol Med, Frankfurt, Germany - Author

Groene Hart Hosp, Dept Internal Med, Gouda, Netherlands - Author

Guys & St Thomas Natl Hlth Serv Fdn Trust, Natl Inst Hlth Res, Biomed Res Ctr, London, England - Author

Hannover Med Sch, Inst Immunol, Hannover, Germany - Author

Hannover Med Sch, MHH, Inst Transplant Immunol, Hannover, Germany - Author

Heidelberg Inst Stem Cell Technol & Expt Med HI S, Heidelberg, Germany - Author

Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH), Heidelberg, Germany. - Author

Heidelberg Univ, Dept Hematol Oncol & Rheumatol, Heidelberg, Germany - Author

Heidelberg Univ, Sect Mol Immunol, Inst Immunol, Heidelberg, Germany - Author

Helmholtz Ctr Infect Res, Dept Vaccinol & Appl Microbiol, Braunschweig, Germany - Author

Helmholtz Ctr Infect Res, Expt Immunol, Braunschweig, Germany - Author

Helmholtz Zentrum Munchen, Inst Innovat Radiotherapy iRT, Expt Immune Biol, Neuherberg, Germany - Author

Henan Univ, Life Sci, Kaifeng, Peoples R China - Author

Hop St Louis, AP HP, Dept Gastroenterol, Paris, France - Author

Houston Methodist Hosp Res Inst, Flow Cytometry, Houston, TX USA - Author

Humanitas Clin & Res Ctr, Flow Cytometry Core, Milan, Italy - Author

Humanitas Clin & Res Ctr, Lab Translat Immunol, Rozzano, Italy - Author

Humanitas Univ, Milan, Italy - Author

Humboldt Univ, Berlin, Germany - Author

ImmuneCarta, Caprion Biosci, Montreal, PQ, Canada - Author

Immunology Section, Lund University, Lund, Sweden. - Author

Infection and Immunity Program and Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia. - Author

Innate Immunty Unit, Department of Immunology, Institut Pasteur, Paris, France. - Author

Inst Biomed Res Salamanca IBSAL, Salamanca, Spain - Author

Inst Pasteur, Dept Immunol, Cellular Lymphocytes Biol, Paris, France - Author

Inst Pasteur, Innate Immunty Unit, Dept Immunol, Paris, France - Author

Inst Pasteur, INSERM, U1223, Paris, France - Author

Inst Pasteur, Unit Lymphopoiesis, Dept Immunol, Paris, France - Author

Institut für Immunologie, Christian-Albrechts-Universität zu Kiel, Kiel, Germany. - Author

Institut für Klinische Molekularbiologie, Christian-Albrechts Universität zu Kiel, Germany. - Author

Institut für Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universität München, Munich, Germany. - Author

Institut für Transfusionsmedizin, Universitätsklinik Schleswig-Holstein, Kiel, Germany. - Author

Institut Pasteur, Inserm U1223, Paris, France. - Author

Institute for Immunology, Faculty of Medicine, Biomedical Center, LMU Munich, Planegg-Martinsried, Germany. - Author

Institute for Systemic Inflammation Research, University of Luebeck, Luebeck, Germany. - Author

Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland. - Author

Institute of Immunology, Centre for Infection Medicine, Department of Veterinary Medicine, Freie Universität Berlin, Germany. - Author

Institute of Immunology, Hannover Medical School, Hannover, Germany. - Author

Institute of Transplant Immunology, Hannover Medical School, MHH, Hannover, Germany. - Author

IRCCS Bambino Gesu Childrens Hosp, Dept Immunol, Rome, Italy - Author

IRCCS Fdn Don Carlo Gnocchi, Milan, Italy - Author

IRCCS Fdn Santa Lucia, Rome, Italy - Author

IRCCS Fondazione Don Carlo Gnocchi, Milan, Italy. - Author

IRCCS Fondazione Santa Lucia, Rome, Italy. - Author

IRCCS Ist Giannina Gaslini, Genoa, Italy - Author

IRCCS Istituto Giannina Gaslini, Genova, Italy. - Author

IRCCS Osped Policlin San Martino, Cell Biol Unit, Genoa, Italy - Author

Isaac and Laura Perlmutter Cancer Center, NYU-Langone Medical Center, New York, USA. - Author

Ist Clin Humanitas IRCCS, Milan, Italy - Author

Ist Italiano Tecnol, Ctr Life Nano Sci Sapienza, Rome, Italy - Author

Ist Sci San Raffaele, Div Immunol Transplantat & Infect Dis, Milan, Italy - Author

Istanbul Univ, Aziz Sancar Inst Expt Med, Dept Immunol, Istanbul, Turkey - Author

Istanbul University, Aziz Sancar Institute of Experimental Medicine, Department of Immunology, Istanbul, Turkey. - Author

Istituto Pasteur - Fondazione Cenci Bolognetti, Rome, Italy. - Author

Jena Univ Hosp, Inst Immunol, Jena, Germany - Author

Johannes Gutenberg Univ Mainz, Univ Med Ctr, Inst Mol Med, Mainz, Germany - Author

Kantonsspital St Gallen, Inst Immunobiol, St Gallen, Switzerland - Author

Karolinska Inst, Biomedicum, Solna, Sweden - Author

Karolinska Inst, Ctr Infect Med, ANA Futura, Dept Med Huddinge, Stockholm, Sweden - Author

Karolinska Univ Hosp, Ctr Mol Med, Solna, Sweden - Author

Karolinska Univ Hosp, Dept Med, Solna, Sweden - Author

Kennedy Institute of Rheumatology, University of Oxford, Oxford, UK. - Author

Kings Coll London, Dept Immunobiol, Sch Immunol & Microbial Sci, London, England - Author

Kings Coll London, London, England - Author

Kings Coll London, Peter Gorer Dept Immunobiol, London, England - Author

Laboratory of Cytomics, Joint Research Unit CIPF-UVEG, Principe Felipe Research Center, Valencia, Spain. - Author

Laboratory of Translational Immunology, Humanitas Clinical and Research Center, Rozzano, Italy. - Author

Leibniz Inst Expt Virol, Dept Virus Immunol, Heinrich Pette Inst, Hamburg, Germany - Author

Leibniz Inst Photon Technol IPHT, Nanobiophoton Dept, Jena, Germany - Author

Leibniz Res Inst Environm Med, Dusseldorf, Germany - Author

Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany. - Author

Leiden Univ, Dept Parasitol, Med Ctr, Leiden, Netherlands - Author

Leiden Univ, Dept Rheumatol, Med Ctr, Leiden, Netherlands - Author

Linkoping Univ, Dept Clin & Expt Medine, Linkoping, Sweden - Author

Ludwig Maximilians Univ Munchen, Core Facil Flow Cytometry, Biomed Ctr, Munich, Germany - Author

Ludwig Maximilians Univ Munchen, Inst Immunol, Biomed Ctr, Fac Med, Planegg Martinsried, Germany - Author

Lund Univ, Immunol Sect, Lund, Sweden - Author

Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Belvaux, Luxembourg. - Author

Luxembourg Inst Hlth, Dept Infect & Immun Expt & Mol Immunol, Esch Sur Alzette, Luxembourg - Author

Luxembourg Inst Hlth, Natl Cytometry Platform, Dept Infect & Immun, Esch Sur Alzette, Luxembourg - Author

Luxembourg Institute of Health, Department of Infection and Immunity, Experimental and Molecular Immunology, Esch-sur-Alzette, Luxembourg. - Author

Macrophage Biology Group, School of Biology, University of Barcelona, Barcelona, Spain. - Author

Mahidol Univ, Siriraj Hosp, Ctr Excellence Flow Cytometry, Fac Med,Dept Res & Dev, Bangkok, Thailand - Author

Max Delbruck Ctr Mol Med, Preparat Flow Cytometry, Berlin, Germany - Author

Max Planck Inst Biol Ageing, FACS, Cologne, Germany - Author

Max Planck Inst Biol Ageing, Imaging Core Facil, Cologne, Germany - Author

Max Planck Inst Infect Biol, Berlin, Germany - Author

MDQuest Ltd, Szeged, Hungary - Author

Med Immunol Campus Erlangen, Erlangen, Germany - Author

Med Univ Graz, Gottfried Schatz Res Ctr, Div Cell Biol Histol & Embryol, Graz, Austria - Author

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Abstract

These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords

Allergy and immunologyCell separationConsensusFlow cytometryHumansPhenotype

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal European Journal Of Immunology due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2019, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Immunology and Allergy.

This publication has been distinguished as a “Highly Cited Paper” by the agencies WoS (ESI, Clarivate) and ESI (Clarivate), meaning that it ranks within the top 1% of the most cited articles in its thematic field during the year of its publication. In terms of the observed impact of the contribution, this work is considered one of the most influential worldwide, as it is recognized as highly cited. (source consulted: ESI Nov 14, 2024)

And this is evidenced by the extremely high normalized impacts through some of the main indicators of this type, which, although dynamic over time and dependent on the set of average global citations at the time of calculation, already indicate that they are well above the average in different agencies:

Normalization of citations relative to the expected citation rate (ESI) by the Clarivate agency: 15.47 (source consulted: ESI Nov 14, 2024)
Weighted Average of Normalized Impact by the Scopus agency: 11.07 (source consulted: FECYT Feb 2024)
Field Citation Ratio (FCR) from Dimensions: 145.28 (source consulted: Dimensions Oct 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-10-17, the following number of citations:

WoS: 461
Scopus: 373
Europe PMC: 370

Impact and social visibility

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Australia; Austria; Belgium; Brazil; Canada; China; Croatia; Czech Republic; Denmark; Estonia; France; Germany; Greece; Hungary; Italy; Japan; Luxembourg; Mexico; Netherlands; New Zealand; Poland; Portugal; Singapore; Sweden; Switzerland; Thailand; Turkey; United Kingdom; United States of America.