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Work in the laboratory of Dieter Adam is supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)-Projektnummer 125440785 - SFB 877, Project B2.

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Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Publicado en:European Journal Of Immunology. 49 (10): 1457-1973 - 2019-10-01 49(10), DOI: 10.1002/eji.201970107

Autores: Cossarizza, A; Chang, HD; Radbruch, A; Acs, A; Adam, D; Adam-Klages, S; Agace, WW; Aghaeepour, N; Akdis, M; Allez, M; Almeida, LN; Alvisi, G; Anderson, G; Andrä, I; Annunziato, F; Anselmo, A; Bacher, P; Baldari, CT; Bari, S; Barnaba, V; Barros-Martins, J; Battistini, L; Bauer, W; Baumgart, S; Baumgarth, N; Baumjohann, D; Baying, B; Bebawy, M; Becher, B; Beisker, W; Benes, V; Beyaert, R; Blanco, A; Boardman, DA; Bogdan, C; Borger, JG; Borsellino, G; Boulais, PE; Bradford, JA; Brenner, D; Brinkman, RR; Brooks, AES; Busch, DH; Büscher, M; Bushnell, TP; Calzetti, F; Cameron, G; Cammarata, I; Cao, X; Cardell, SL; Casola, S; Cassatella, MA; Cavani, A; Celada, A; Chatenoud, L; Chattopadhyay, PK; Chow, S; Christakou, E; Cicin-Sain, L; Clerici, M; Colombo, FS; Cook, L; Cooke, A; Cooper, AM; Corbett, AJ; Cosma, A; Cosmi, L; Coulie, PG; Cumano, A; Cvetkovic, L; Dang, VD; Dang-Heine, C; Davey, MS; Davies, D; De Biasi, S; Del Zotto, G; Dela Cruz, GV; Delacher, M; Della Bella, S; Dellabona, P; Deniz, G; Dessing, M; Di Santo, JP; Diefenbach, A; Dieli, F; Dolf, A; Dörner, T; Dress, RJ; Dudziak, D; Dustin, M; Dutertre, CA; Ebner, F; Eckle, SBG; Edinger, M; Eede, P; Ehrhardt, GRA; Eich, M; Engel, P; Engelhardt, B; Erdei, A; Esser, C; Everts, B; Evrard, M; Falk, CS; Fehniger, TA; Felipo-Benavent, M; Ferry, H; Feuerer, M; Filby, A; Filkor, K; Fillatreau, S; Follo, M; Förster, I; Foster, J; Foulds, GA; Frehse, B; Frenette, PS; Frischbutter, S; Fritzsche, W; Galbraith, DW; Gangaev, A; Garbi, N; Gaudilliere, B; Gazzinelli, RT; Geginat, J; Gerner, W; Gherardin, NA; Ghoreschi, K; Gibellini, L; Ginhoux, F; Goda, K; Godfrey, DI; Goettlinger, C; González-Navajas, JM; Goodyear, CS; Gori, A; Grogan, JL; Grummitt, D; Grützkau, A; Haftmann, C; Hahn, J; Hammad, H; Hämmerling, G; Hansmann, L; Hansson, G; Harpur, CM; Hartmann, S; Hauser, A; Hauser, AE; Haviland, DL; Hedley, D; Hernández, DC; Herrera, G; Herrmann, M; Hess, C; Höfer, T; Hoffmann, P; Hogquist, K; Holland, T; Höllt, T; Holmdahl, R; Hombrink, P; Houston, JP; Hoyer, BF; Huang, B; Huang, FP; Huber, JE; Huehn, J; Hundemer, M; Hunter, CA; Hwang, WYK; Iannone, A; Ingelfinger, F; Ivison, SM; Jäck, HM; Jani, PK; Jávega, B; Jonjic, S; Kaiser, T; Kalina, T; Kamradt, T; Kaufmann, SHE; Keller, B; Ketelaars, SLC; Khalilnezhad, A; Khan, S; Kisielow, J; Klenerman, P; Knopf, J; Koay, HF; Kobow, K; Kolls, JK; Kong, WT; Kopf, M; Korn, T; Kriegsmann, K; Kristyanto, H; Kroneis, T; Krueger, A; Kühne, J; Kukat, C; Kunkel, D; Kunze-Schumacher, H; Kurosaki, T; Kurts, C; Kvistborg, P; Kwok, I; Landry, J; Lantz, O; Lanuti, P; LaRosa, F; Lehuen, A; LeibundGut-Landmann, S; Leipold, MD; Leung, LYT; Levings, MK; Lino, AC; Liotta, F; Litwin, V; Liu, Y; Ljunggren, HG; Lohoff, M; Lombardi, G; Lopez, L; López-Botet, M; Lovett-Racke, AE; Lubberts, E; Luche, H; Ludewig, B; Lugli, E; Lunemann, S; Maecker, HT; Maggi, L; Maguire, O; Mair, F; Mair, KH; Mantovani, A; Manz, RA; Marshall, AJ; Martínez-Romero, A; Martrus, G; Marventano, I; Maslinski, W; Matarese, G; Mattioli, AV; Maueröder, C; Mazzoni, A; McCluskey, J; McGrath, M; McGuire, HM; McInnes, IB; Mei, HE; Melchers, F; Melzer, S; Mielenz, D; Miller, SD; Mills, KHG; Minderman, H; Mjösberg, J; Moore, J; Moran, B; Moretta, L; Mosmann, TR; Müller, S; Multhoff, G; Muñoz, LE; Münz, C; Nakayama, T; Nasi, M; Neumann, K; Ng, LG; Niedobitek, A; Nourshargh, S; Núñez, G; O'Connor, JE; Ochel, A; Oja, A; Ordonez, D; Orfao, A; Orlowski-Oliver, E; Ouyang, W; Oxenius, A; Palankar, R; Panse, I; Pattanapanyasat, K; Paulsen, M; Pavlinic, D; Penter, L; Peterson, P; Peth, C; Petriz, J; Piancone, F; Pickl, WF; Piconese, S; Pinti, M; Pockley, AG; Podolska, MJ; Poon, Z; Pracht, K; Prinz, I; Pucillo, CEM; Quataert, SA; Quatrini, L; Quinn, KM; Radbruch, H; Radstake, TRDJ; Rahmig, S; Rahn, HP; Rajwa, B; Ravichandran, G; Raz, Y; Rebhahn, JA; Recktenwald, D; Reimer, D; Reis e Sousa, C; Remmerswaal, EBM; Richter, L; Rico, LG; Riddell, A; Rieger, AM; Robinson, JP; Romagnani, C; Rubartelli, A; Ruland, J; Saalmüller, A; Saeys, Y; Saito, T; Sakaguchi, S; Sala-de-Oyanguren, F; Samstag, Y; Sanderson, S; Sandrock, I; Santoni, A; Sanz, RB; Saresella, M; Sautes-Fridman, C; Sawitzki, B; Schadt, L; Scheffold, A; Scherer, HU; Schiemann, M; Schildberg, FA; Schimisky, E; Schlitzer, A; Schlosser, J; Schmid, S; Schmitt, S; Schober, K; Schraivogel, D; Schuh, W; Schüler, T; Schulte, R; Schulz, AR; Schulz, SR; Scottá, C; Scott-Algara, D; Sester, DP; Shankey, TV; Silva-Santos, B; Simon, AK; Sitnik, KM; Sozzani, S; Speiser, DE; Spidlen, J; Stahlberg, A; Stall, AM; Stanley, N; Stark, R; Stehle, C; Steinmetz, T; Stockinger, H; Takahama, Y; Takeda, K; Tan, L; Tárnok, A; Tiegs, G; Toldi, G; Tornack, J; Traggiai, E; Trebak, M; Tree, TIM; Trotter, J; Trowsdale, J; Tsoumakidou, M; Ulrich, H; Urbanczyk, S; van de Veen, W; van den Broek, M; van der Pol, E; Van Gassen, S; Van Isterdael, G; van Lier, RAW; Veldhoen, M; Vento-Asturias, S; Vieira, P; Voehringer, D; Volk, HD; von Borstel, A; von Volkmann, K; Waisman, A; Walker, RV; Wallace, PK; Wang, SA; Wang, XM; Ward, MD; Ward-Hartstonge, KA; Warnatz, K; Warnes, G; Warth, S; Waskow, C; Watson, JV; Watzl, C; Wegener, L; Weisenburger, T; Wiedemann, A; Wienands, J; Wilharm, A; Wilkinson, RJ; Willimsky, G; Wing, JB; Winkelmann, R; Winkler, TH; Wirz, OF; Wong, A; Wurst, P; Yang, JHM; Yang, JH; Yazdanbakhsh, M; Yu, LP; Yue, A; Zhang, HL; Zhao, Y; Ziegler, SM; Zielinski, C; Zimmermann, J; Zychlinsky, A

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Resumen

These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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